| Gene | Mpg |
| Chromosome | 11 |
| Deletion Start | 32,232,035 |
| Deletion End | 32,226,544 |
| Deletion Size | 5,492 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,227,346 | 32,227,364 | GAGCAGTGCCCAGACAAAC |
| TUP | 32,227,370 | 32,227,394 | ATGTTTCTCTGATAGGCCCTGCCCA |
| TUR | 32,227,458 | 32,227,481 | CAATCACACCATCTCCAAGGAAAC |
| TDF | 32,231,304 | 32,231,322 | ATGCCAGACTCGCCCACAC |
| TDP | 32,231,326 | 32,231,351 | TTGATCACAGTTCTGGCTCTGAGGGC |
| TDR | 32,231,382 | 32,231,401 | ACCTTGGGAAGCCTCCACTC |
PCR Primers
| Primer | Sequence |
| SU | CAGACTGAGATTCCCAAGGC |
| SD | TTGCTGAGCTGGAGAAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 136 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17409A-H8 | VGB6 | C57BL/6NTac | |
| 17409A-B6 | VGB6 | C57BL/6NTac | |
| 17409A-D11 | VGB6 | C57BL/6NTac | |
| 17409A-B10 | VGB6 | C57BL/6NTac | |
| 17409A-G11 | VGB6 | C57BL/6NTac | |
| 17409A-B1 | VGB6 | C57BL/6NTac | |
