| Gene | Mrvi1 |
| Chromosome | 7 |
| Deletion Start | 110,945,889 |
| Deletion End | 110,921,319 |
| Deletion Size | 24,571 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,945,774 | 110,945,795 | GGGCCAAACTAAGCATTCTGTC |
| TUP | 110,945,750 | 110,945,773 | TGAGGCCACAGCTTACAGACTGGC |
| TUR | 110,945,686 | 110,945,705 | TCAGAGGGAAAGCCACACAC |
| TDF | 110,922,259 | 110,922,281 | AGTCTTGGTCTTCTGTGATGTTC |
| TDP | 110,922,230 | 110,922,258 | TGGGCTGGAGGCTTAGAAAGATGAACAAT |
| TDR | 110,922,205 | 110,922,224 | ATTGGAGGGTGGGAGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | ACAAAGCCAGAAAGCAGGAG |
| SD | GCAATGTCAAAAGTTCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17411A-E10 | VGB6 | C57BL/6NTac | |
| 17411A-C12 | VGB6 | C57BL/6NTac | |
| 17411A-E7 | VGB6 | C57BL/6NTac | |
| 17411A-D1 | VGB6 | C57BL/6NTac | |
| 17411A-B9 | VGB6 | C57BL/6NTac | |
| 17411A-D4 | VGB6 | C57BL/6NTac | |
