| Gene | Mtch2 |
| Chromosome | 2 |
| Deletion Start | 90,865,306 |
| Deletion End | 90,847,302 |
| Deletion Size | 18,005 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,847,332 | 90,847,349 | TCCGGTCTCACCATCCTG |
| TUP | 90,847,351 | 90,847,375 | CCCAGCCGCTCATGTACGTGAAAGT |
| TUR | 90,847,386 | 90,847,406 | GGAAAGGGCGACGACTCATAC |
| TDF | 90,864,777 | 90,864,795 | GCGGTTCCCATTCCCATTC |
| TDP | 90,864,796 | 90,864,821 | CCAAGCAATGCCCTGTTGTAGGAGGA |
| TDR | 90,864,839 | 90,864,858 | GGTCTGTCTGGCACCTCAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGGACCTGCAGAATTCAC |
| SD | TGAAAGGTAAGATGGGTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17412A-A5 | VGB6 | C57BL/6NTac | |
| 17412A-B12 | VGB6 | C57BL/6NTac | |
| 17412A-C3 | VGB6 | C57BL/6NTac | |
| 17412A-D2 | VGB6 | C57BL/6NTac | |
| 17412A-G3 | VGB6 | C57BL/6NTac | |
