| Gene | Mtmr4 |
| Chromosome | 11 |
| Deletion Start | 87,614,319 |
| Deletion End | 87,593,434 |
| Deletion Size | 20,886 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,594,211 | 87,594,228 | CTCCCAGGTGTTGCAGTC |
| TUP | 87,594,230 | 87,594,255 | ATTAAGCCACTGCAGGTGACAGCTGA |
| TUR | 87,594,277 | 87,594,295 | GAGGACCCACCGTCAGAAG |
| TDF | 87,613,675 | 87,613,696 | CCATCCTTGCCAGTTTCCTTTG |
| TDP | 87,613,701 | 87,613,727 | ACTTTCCGGATTGCAGTGTTGTTCTCC |
| TDR | 87,613,762 | 87,613,782 | CAGCGAGTCACCTACCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GTGCTCTACTGCTGGGTGTG |
| SD | TGCACAGACACAGAGCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17415A-A4 | VGB6 | C57BL/6NTac | |
| 17415A-B5 | VGB6 | C57BL/6NTac | |
| 17415A-D4 | VGB6 | C57BL/6NTac | |
| 17415A-D10 | VGB6 | C57BL/6NTac | |
| 17415A-D12 | VGB6 | C57BL/6NTac | |
| 17415A-H12 | VGB6 | C57BL/6NTac | |
