| Gene | Mus81 |
| Chromosome | 19 |
| Deletion Start | 5,488,119 |
| Deletion End | 5,482,903 |
| Deletion Size | 5,217 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,487,886 | 5,487,905 | CTTGTGTTCGCCAGGCATTG |
| TUP | 5,487,861 | 5,487,882 | TCCCTCCAACGGTACCCGCTAC |
| TUR | 5,487,816 | 5,487,835 | CTCCGAAGTGCTGGAGTATC |
| TDF | 5,483,394 | 5,483,415 | ACCAGTGCTCCTGGAGACTGAG |
| TDP | 5,483,373 | 5,483,393 | CTTGGCTCTGCGTGGGCACCT |
| TDR | 5,483,335 | 5,483,353 | CCCTCCCTCCTTGGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | GCAAGCCAAGTTCAGGTTTC |
| SD | TTTAGGAATGGGTAAGGACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17416A-H9 | VGB6 | C57BL/6NTac | |
| 17416A-B12 | VGB6 | C57BL/6NTac | |
| 17416A-F8 | VGB6 | C57BL/6NTac | |
| 17416A-E10 | VGB6 | C57BL/6NTac | |
| 17416A-A7 | VGB6 | C57BL/6NTac | |
