| Gene | Napb |
| Chromosome | 2 |
| Deletion Start | 148,732,360 |
| Deletion End | 148,697,324 |
| Deletion Size | 35,037 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,732,168 | 148,732,187 | GCGCAACTGCTTAGGGTTAC |
| TUP | 148,732,142 | 148,732,164 | ATTCCCAGTGGCCCACGCTCTGT |
| TUR | 148,732,099 | 148,732,117 | GCCCAAGGGATGCGATGAG |
| TDF | 148,697,492 | 148,697,513 | AAACCAGGTGAAGGAGTTTGAC |
| TDP | 148,697,461 | 148,697,486 | ATCACGTTTGGATCAGTGGCTGACCA |
| TDR | 148,697,426 | 148,697,449 | CCCTTGGATGGACTTCTTGATACG |
PCR Primers
| Primer | Sequence |
| SU | TCTACCTCCAAGGGTCCAAG |
| SD | GCTATAAAGGAAAGCCAAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17421B-A2 | VGB6 | C57BL/6NTac | |
| 17421B-A8 | VGB6 | C57BL/6NTac | |
| 17421B-A11 | VGB6 | C57BL/6NTac | |
| 17421B-A12 | VGB6 | C57BL/6NTac | |
| 17421B-D7 | VGB6 | C57BL/6NTac | |
| 17421B-F6 | VGB6 | C57BL/6NTac | |
