| Gene | Ncaph |
| Chromosome | 2 |
| Deletion Start | 127,127,714 |
| Deletion End | 127,104,168 |
| Deletion Size | 23,547 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,127,529 | 127,127,548 | GACGCTCCAGGGTCTTTGAC |
| TUP | 127,127,492 | 127,127,521 | TCAGTACAGATTCGATTCATCTGGCCTCCC |
| TUR | 127,127,470 | 127,127,491 | CAGAGCTGCACTCACCTGTTAG |
| TDF | 127,104,636 | 127,104,657 | AGGTCTCACCACTGCACCTTTG |
| TDP | 127,104,597 | 127,104,625 | ACAGTCTTCTCTTGGCAGTCAGTTAGCCC |
| TDR | 127,104,553 | 127,104,571 | GGCTGCTACACCTCTCATC |
PCR Primers
| Primer | Sequence |
| SU | GCCTTGCCATTCATATACCC |
| SD | ACTGGGCACTCAGACACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17423B-D5 | VGB6 | C57BL/6NTac | |
| 17423C-B9 | VGB6 | C57BL/6NTac | |
