| Gene | Ndufs3 |
| Chromosome | 2 |
| Deletion Start | 90,904,644 |
| Deletion End | 90,894,702 |
| Deletion Size | 9,943 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,904,333 | 90,904,355 | GTTTGTCCTTGCCTTGGAATGTC |
| TUP | 90,904,305 | 90,904,330 | TCCCTCCTGGCAAATCTCTGCTTGTC |
| TUR | 90,904,260 | 90,904,283 | GTGCTCGATTCTAACAGCAAAGTG |
| TDF | 90,894,953 | 90,894,976 | ACAGAAGCCAAGGGAAAGGGATAG |
| TDP | 90,894,925 | 90,894,950 | CCTGGAGGAATCTGCACCTAGGCTGG |
| TDR | 90,894,892 | 90,894,913 | GTTAGGGCTGATGACTGAGGAG |
PCR Primers
| Primer | Sequence |
| SU | GGAAGTCCATAACCCTGTGC |
| SD | CCATCCCCACTAAATAAATGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17424A-A7 | VGB6 | C57BL/6NTac | |
| 17424A-B6 | VGB6 | C57BL/6NTac | |
| 17424A-B8 | VGB6 | C57BL/6NTac | |
| 17424A-H10 | VGB6 | C57BL/6NTac | |
| 17424A-F7 | VGB6 | C57BL/6NTac | |
