| Gene | Sirt6 |
| Chromosome | 10 |
| Deletion Start | 81,627,534 |
| Deletion End | 81,622,311 |
| Deletion Size | 5,224 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,626,781 | 81,626,801 | CAGGAACAGCACTCTGGGAAG |
| TUP | 81,626,755 | 81,626,775 | TCGGCATGTGCAAAGGCCCTG |
| TUR | 81,626,718 | 81,626,740 | TCCAGCCACACTCTTAACACATC |
| TDF | 81,622,959 | 81,622,982 | CAAGACTGAATCTCAGCTCCCAAG |
| TDP | 81,622,936 | 81,622,958 | TGTTTCTCCAGTGCCTGGTGGGT |
| TDR | 81,622,913 | 81,622,931 | CTCAGGTTCCAGCCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | CCTGTGAGTGGCCTCATCAC |
| SD | GTGTGTGAGACTGCTGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17425A-A4 | VGB6 | C57BL/6NTac | |
| 17425A-A8 | VGB6 | C57BL/6NTac | |
| 17425A-C3 | VGB6 | C57BL/6NTac | |
| 17425A-H1 | VGB6 | C57BL/6NTac | |
| 17425A-D6 | VGB6 | C57BL/6NTac | |
| 17425A-D9 | VGB6 | C57BL/6NTac | |
