| Gene | Nek9 |
| Chromosome | 12 |
| Deletion Start | 85,339,218 |
| Deletion End | 85,301,774 |
| Deletion Size | 37,445 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,338,927 | 85,338,946 | CCGCTTTCCAGTGCTGCTTG |
| TUP | 85,338,902 | 85,338,924 | CCTGGCCCTCTATGGTCAGGCGT |
| TUR | 85,338,822 | 85,338,844 | TGACAGCGGTGATTAAACCAATG |
| TDF | 85,302,021 | 85,302,038 | GCCAAGCCTTCCGTAGAC |
| TDP | 85,301,995 | 85,302,020 | TGACAGCAAACCCTGAGAGGTCAGAC |
| TDR | 85,301,931 | 85,301,954 | GCAGACAAAGCAGTTCCACATTAG |
PCR Primers
| Primer | Sequence |
| SU | CCGCTTCCACGACACTCG |
| SD | CTCTTCAAAGGTGGGCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17427A-C4 | VGB6 | C57BL/6NTac | |
| 17427A-C9 | VGB6 | C57BL/6NTac | |
| 17427A-E2 | VGB6 | C57BL/6NTac | |
| 17427A-F11 | VGB6 | C57BL/6NTac | |
| 17427A-G10 | VGB6 | C57BL/6NTac | |
| 17427A-F3 | VGB6 | C57BL/6NTac | |
