| Gene | Nmral1 |
| Chromosome | 16 |
| Deletion Start | 4,718,931 |
| Deletion End | 4,711,408 |
| Deletion Size | 7,524 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,718,889 | 4,718,906 | TTGGAGCCACAGGTGAGC |
| TUP | | | CGGTCCTCCGGACCTT |
| TUR | 4,718,844 | 4,718,867 | ACCACAGCCGTTCTTCCTACAAAC |
| TDF | 4,711,674 | 4,711,694 | CCCTGGCTTTCTAGGACTCTG |
| TDP | 4,711,642 | 4,711,665 | CAGTCTTCCTGGCATCACCAAGGC |
| TDR | 4,711,618 | 4,711,640 | TCCACCATGTCTGTCTCCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTGCACAGAATTGCATCC |
| SD | ATGCTCAGGGTTGAGAGTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17431A-B11 | VGB6 | C57BL/6NTac | |
| 17431A-B2 | VGB6 | C57BL/6NTac | |
| 17431A-H2 | VGB6 | C57BL/6NTac | |
| 17431A-G6 | VGB6 | C57BL/6NTac | |
| 17431A-E8 | VGB6 | C57BL/6NTac | |
| 17431A-F10 | VGB6 | C57BL/6NTac | |
