| Gene | Npl |
| Chromosome | 1 |
| Deletion Start | 153,545,327 |
| Deletion End | 153,515,244 |
| Deletion Size | 30,084 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,544,721 | 153,544,740 | TGCTGAAGAGATGGCTTGAC |
| TUP | 153,544,697 | 153,544,720 | TGGAACCATGCTTGCTGCACAAGA |
| TUR | 153,544,675 | 153,544,696 | TGATCTGTACCTGGGTCCTTAC |
| TDF | 153,515,539 | 153,515,557 | TCTGAAGTTCGCGCTGAGG |
| TDP | 153,515,510 | 153,515,535 | TGCTGGACGGGATTCAGGACAAGATC |
| TDR | 153,515,461 | 153,515,482 | CGAAATCCAAGAGGTCCGTGTC |
PCR Primers
| Primer | Sequence |
| SU | ACCTTTGCTGCTGGATCTTC |
| SD | CATCAATATTCCAATCCTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17432A-B2 | VGB6 | C57BL/6NTac | |
| 17432A-C3 | VGB6 | C57BL/6NTac | |
