| Gene | Nr1h3 |
| Chromosome | 2 |
| Deletion Start | 91,193,656 |
| Deletion End | 91,184,275 |
| Deletion Size | 9,382 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,192,753 | 91,192,773 | ACCCATTCTCCCGTGCTTCTC |
| TUP | 91,192,728 | 91,192,751 | TGGGCTCAGATTCTGCAACGGAGT |
| TUR | 91,192,691 | 91,192,710 | CCCTGGTCTCCTGCATCTTG |
| TDF | 91,185,132 | 91,185,154 | AAACCTGGAGACCTGTTAGTGAC |
| TDP | 91,185,101 | 91,185,129 | CCTAGCCTGAGGAAGTTGAGTTACAGGGT |
| TDR | 91,185,081 | 91,185,100 | ATGGCGGGAGCAGGAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGAGACTCCTGAATTCAGC |
| SD | CTTGGCCTTCCTGTTTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17433A-C12 | VGB6 | C57BL/6NTac | |
| 17433A-G3 | VGB6 | C57BL/6NTac | |
| 17433A-A7 | VGB6 | C57BL/6NTac | |
| 17433A-B9 | VGB6 | C57BL/6NTac | |
