| Gene | Nt5dc3 |
| Chromosome | 10 |
| Deletion Start | 86,812,377 |
| Deletion End | 86,779,113 |
| Deletion Size | 33,265 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,779,984 | 86,780,004 | TGCTTTGTGCTTCTTGCAGTC |
| TUP | 86,780,007 | 86,780,033 | ACGTTTGGAACCTGGAGTAAAGGGCCA |
| TUR | 86,780,060 | 86,780,078 | CCCAAATCCCAGTGTCCTC |
| TDF | 86,812,129 | 86,812,151 | GCATTAAAGGCTAGGCTCACAAC |
| TDP | 86,812,160 | 86,812,184 | TCAAGGGTCGCAGCATTAGGAAGGT |
| TDR | 86,812,188 | 86,812,210 | ACGTTTCCTTAACACAGTGGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTGACTCCGCCTTTGACTC |
| SD | CGAAGACAGAGATATAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17434A-A9 | VGB6 | C57BL/6NTac | |
| 17434A-B7 | VGB6 | C57BL/6NTac | |
| 17434A-G1 | VGB6 | C57BL/6NTac | |
| 17434A-G2 | VGB6 | C57BL/6NTac | |
| 17434A-C9 | VGB6 | C57BL/6NTac | |
