| Gene | Orc3 |
| Chromosome | 4 |
| Deletion Start | 34,614,906 |
| Deletion End | 34,584,809 |
| Deletion Size | 30,098 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,614,075 | 34,614,098 | GAATATCAAACAGGGCAGCAGATG |
| TUP | 34,614,040 | 34,614,068 | AAAGGTCTATTGGTCTGTGCTGCTATGCC |
| TUR | 34,613,980 | 34,614,000 | TCAGCTTCCTTCAGGAGTGTC |
| TDF | 34,585,392 | 34,585,415 | TGGCTTTGTTCTGAAAGTTAGAGC |
| TDP | 34,585,365 | 34,585,391 | TGGAAATAGCCACGTGAATCCCAGTGC |
| TDR | 34,585,340 | 34,585,361 | ACAGCACAAGTACAGAGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | ATCCAATGGTCTCCAAGTCC |
| SD | CTTCAGGGCCGAATGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17435A-A3 | VGB6 | C57BL/6NTac | |
| 17435A-A7 | VGB6 | C57BL/6NTac | |
| 17435A-E7 | VGB6 | C57BL/6NTac | |
| 17435A-F10 | VGB6 | C57BL/6NTac | |
| 17435A-H1 | VGB6 | C57BL/6NTac | |
| 17435A-G4 | VGB6 | C57BL/6NTac | |
