| Gene | Osgepl1 |
| Chromosome | 1 |
| Deletion Start | 53,323,248 |
| Deletion End | 53,314,882 |
| Deletion Size | 8,367 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,315,384 | 53,315,407 | CCTTGTCTTGGCTTTTAAGGCTTC |
| TUP | 53,315,408 | 53,315,437 | CCCAGTTCACAGTAGGGTCTCAACATTTGC |
| TUR | 53,315,450 | 53,315,472 | GGAATGGTTGTTGCTTCCTTCTG |
| TDF | 53,322,453 | 53,322,474 | AATACTGCCTAGTCCAGGTGTC |
| TDP | 53,322,475 | 53,322,497 | CCCTTGTGGCAGTGTCAAGGCTC |
| TDR | 53,322,507 | 53,322,531 | CAAGGTAAGACTTTAAGCAGACCAC |
PCR Primers
| Primer | Sequence |
| SU | ACTCAGGTGGTAGCGTGC |
| SD | CACTGGTGCACGTGCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17436A-H6 | VGB6 | C57BL/6NTac | |
| 17436A-B3 | VGB6 | C57BL/6NTac | |
| 17436A-B6 | VGB6 | C57BL/6NTac | |
| 17436A-G1 | VGB6 | C57BL/6NTac | |
| 17436A-H2 | VGB6 | C57BL/6NTac | |
| 17436A-E6 | VGB6 | C57BL/6NTac | |
