| Gene | Pacsin3 |
| Chromosome | 2 |
| Deletion Start | 91,264,319 |
| Deletion End | 91,260,211 |
| Deletion Size | 4,109 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,260,483 | 91,260,502 | AACGTGCCCGCATTGAGAAG |
| TUP | 91,260,504 | 91,260,523 | CCTACGCCCAGCAGCTGGCT |
| TUR | 91,260,592 | 91,260,613 | GGTCCCACCCTCATAACCTTTG |
| TDF | 91,263,954 | 91,263,974 | GACGAAGAAAGCCAGGGACTC |
| TDP | 91,263,977 | 91,263,999 | TCCTCACAGGCTGCTCAGGGTCC |
| TDR | 91,264,081 | 91,264,101 | GGCCGACAAACTGGAGGATAC |
PCR Primers
| Primer | Sequence |
| SU | ACCAGTCTGGCATTCTCAGC |
| SD | CTCCCACCAGCAAGAGTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17439A-B10 | VGB6 | C57BL/6NTac | |
| 17439A-C1 | VGB6 | C57BL/6NTac | |
| 17439A-D1 | VGB6 | C57BL/6NTac | |
| 17439A-H6 | VGB6 | C57BL/6NTac | |
| 17439A-E1 | VGB6 | C57BL/6NTac | |
| 17439A-G4 | VGB6 | C57BL/6NTac | |
