| Gene | Pak2 |
| Chromosome | 16 |
| Deletion Start | 32,052,359 |
| Deletion End | 32,020,176 |
| Deletion Size | 32,184 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,052,333 | 32,052,357 | TGATAACGGAGAGCTAGAAGACAAG |
| TUP | 32,052,309 | 32,052,331 | CCCCAGCACCTCCAGTTCGGATG |
| TUR | 32,052,226 | 32,052,247 | TCCTCTGGAACAGAAGGCAAAG |
| TDF | 32,020,249 | 32,020,270 | GCAGCATCCTTTCCTGAAACTG |
| TDP | 32,020,221 | 32,020,247 | CCAAACCATTGTCTAGCTTGACGCCAC |
| TDR | 32,020,178 | 32,020,200 | TGTTAGCGGTTACTCTTCATTGC |
PCR Primers
| Primer | Sequence |
| SU | GGAATCATCTCAGAGTTGTTAGG |
| SD | AAGGGAAGGTCACGAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17440A-B11 | VGB6 | C57BL/6NTac | |
| 17440A-A10 | VGB6 | C57BL/6NTac | |
