| Gene | Parl |
| Chromosome | 16 |
| Deletion Start | 20,302,324 |
| Deletion End | 20,279,944 |
| Deletion Size | 22,381 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,302,105 | 20,302,126 | GCTCCCGCTTTCCATTGTTTAC |
| TUP | 20,302,074 | 20,302,100 | TCTTTGCTAAAGCTGACTCCTCCCTGC |
| TUR | 20,302,029 | 20,302,047 | GGCACCCAGAAGCCAGTAG |
| TDF | 20,280,710 | 20,280,729 | TTCCACAAAGCCTCACAAAC |
| TDP | 20,280,678 | 20,280,705 | TTCAATTCCTGGGACCTACGTGATGTCC |
| TDR | 20,280,642 | 20,280,662 | AGGCACAAGTACCACAGTCAG |
PCR Primers
| Primer | Sequence |
| SU | CCACTGTACAGGGCCATTTC |
| SD | CTGCCAGAGGAGCCAGTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17441A-F11 | VGB6 | C57BL/6NTac | |
| 17441A-D5 | VGB6 | C57BL/6NTac | |
| 17441A-B6 | VGB6 | C57BL/6NTac | |
| 17441A-D4 | VGB6 | C57BL/6NTac | |
| 17441A-A12 | VGB6 | C57BL/6NTac | |
| 17441A-C9 | VGB6 | C57BL/6NTac | |
