| Gene | Parp1 |
| Chromosome | 1 |
| Deletion Start | 180,600,573 |
| Deletion End | 180,569,037 |
| Deletion Size | 31,537 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,569,737 | 180,569,757 | GCAGCTATCTGGTCTCTTCAC |
| TUP | 180,569,758 | 180,569,780 | TGACGCTTCCGAGATGGTCAGGA |
| TUR | 180,569,789 | 180,569,807 | AACGCCAGGACCCGTCATC |
| TDF | 180,599,635 | 180,599,654 | GGGCAAGCACAGTGTCAAAG |
| TDP | 180,599,656 | 180,599,679 | TACTGTCCCTCCAGCCTGACACGG |
| TDR | 180,599,680 | 180,599,702 | AAATGAACCTCCACCCGAATCTG |
PCR Primers
| Primer | Sequence |
| SU | TGAGGTGGAATTATTTGGGC |
| SD | AGGCAGACATCGTGACTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17442A-A11 | VGB6 | C57BL/6NTac | |
| 17442A-B9 | VGB6 | C57BL/6NTac | |
| 17442A-D3 | VGB6 | C57BL/6NTac | |
| 17442A-H5 | VGB6 | C57BL/6NTac | |
| 17442A-H3 | VGB6 | C57BL/6NTac | |
| 17442A-G2 | VGB6 | C57BL/6NTac | |
