| Gene | Pcnxl3 |
| Chromosome | 19 |
| Deletion Start | 5,688,146 |
| Deletion End | 5,665,021 |
| Deletion Size | 23,126 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,688,009 | 5,688,033 | GATCTTCTTGCTCATCTTTCCCTTC |
| TUP | 5,687,981 | 5,688,004 | TGTACATGGTGAGAGGCTGCCACC |
| TUR | 5,687,936 | 5,687,955 | TGCCCATCCCTCCAGACTAC |
| TDF | 5,665,743 | 5,665,763 | GACCTCCCTCAGCAATCATCC |
| TDP | 5,665,720 | 5,665,741 | CCCTTGGCACACCCTACGCCTG |
| TDR | 5,665,689 | 5,665,707 | TCCAGCTTTGCCCGCTTAC |
PCR Primers
| Primer | Sequence |
| SU | CTCGGCGCTTTACAACAG |
| SD | CCTCTACCAACCTCACCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17444A-C2 | VGB6 | C57BL/6NTac | |
| 17444A-F6 | VGB6 | C57BL/6NTac | |
| 17444A-G6 | VGB6 | C57BL/6NTac | |
| 17444A-G9 | VGB6 | C57BL/6NTac | |
| 17444A-H4 | VGB6 | C57BL/6NTac | |
| 17444A-H9 | VGB6 | C57BL/6NTac | |
