| Gene | Pcx |
| Chromosome | 19 |
| Deletion Start | 4,621,322 |
| Deletion End | 4,601,603 |
| Deletion Size | 19,720 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,602,236 | 4,602,255 | TGGCTATGGGTTCCTCTCAG |
| TUP | 4,602,258 | 4,602,279 | CGAGCAGACTTTGCCCAGGCCT |
| TUR | 4,602,282 | 4,602,301 | AACCGGACTCCAGCATCTTG |
| TDF | 4,620,940 | 4,620,960 | AAAGACACCCAGGCCATGAAG |
| TDP | 4,620,964 | 4,620,987 | TAGTGCCACCAGAGCCAGTCAGGT |
| TDR | 4,621,011 | 4,621,034 | GACAGGACAGAGACGACATAAGAC |
PCR Primers
| Primer | Sequence |
| SU | GAGGTTCGTGGAGAATGAGG |
| SD | TTGAGCAAATTTCCCAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17445A-A6 | VGB6 | C57BL/6NTac | |
| 17445A-B11 | VGB6 | C57BL/6NTac | |
| 17445A-H9 | VGB6 | C57BL/6NTac | |
| 17445A-D2 | VGB6 | C57BL/6NTac | |
| 17445A-D7 | VGB6 | C57BL/6NTac | |
| 17445A-D6 | VGB6 | C57BL/6NTac | |
