| Gene | Pcyt1a |
| Chromosome | 16 |
| Deletion Start | 32,471,507 |
| Deletion End | 32,451,718 |
| Deletion Size | 19,790 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,452,108 | 32,452,129 | CCGGCTAGTGATTTGGAATGTG |
| TUP | 32,452,131 | 32,452,158 | CCTGTGTTCTTCGGTCCTTCCTATGTCA |
| TUR | 32,452,162 | 32,452,182 | GTGCCCAGCAGTAAAGTGAAG |
| TDF | 32,470,979 | 32,470,999 | GCGTTTGGACTGCACACTAAG |
| TDP | 32,471,001 | 32,471,028 | AGCCCTTAGATGCCTCTGACAGAAGTCA |
| TDR | 32,471,041 | 32,471,060 | GGTCTCCACCTGCTCACAAG |
PCR Primers
| Primer | Sequence |
| SU | GTCCCAACTGGTCACAAAC |
| SD | CATTCCACGCTTCCTTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17446A-B6 | VGB6 | C57BL/6NTac | |
| 17446A-G2 | VGB6 | C57BL/6NTac | |
| 17446A-B10 | VGB6 | C57BL/6NTac | |
| 17446A-D11 | VGB6 | C57BL/6NTac | |
