| Gene | Pex16 |
| Chromosome | 2 |
| Deletion Start | 92,381,122 |
| Deletion End | 92,375,567 |
| Deletion Size | 5,556 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,375,711 | 92,375,730 | TCTGACCCAGGAACCCTATG |
| TUP | 92,375,732 | 92,375,755 | CTCTTTGCCTGCTGACCCAGGGTA |
| TUR | 92,375,768 | 92,375,790 | CAGGAACCCAGACAGAACTGAAG |
| TDF | 92,380,402 | 92,380,422 | CCAATCAGAGCCAAGATCCTC |
| TDP | 92,380,425 | 92,380,448 | CCTGCTCCAGCTGCTTACAGACCA |
| TDR | 92,380,475 | 92,380,496 | CACGTTGTCCTACCACACTTAC |
PCR Primers
| Primer | Sequence |
| SU | CAAGACAGCCAACCAAGGAG |
| SD | GCCCAGTTCAGCTTGTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17448A-A9 | VGB6 | C57BL/6NTac | |
| 17448A-B3 | VGB6 | C57BL/6NTac | |
| 17448A-B10 | VGB6 | C57BL/6NTac | |
| 17448A-F8 | VGB6 | C57BL/6NTac | |
| 17448A-H12 | VGB6 | C57BL/6NTac | |
