| Gene | Pfkl |
| Chromosome | 10 |
| Deletion Start | 78,009,680 |
| Deletion End | 77,988,150 |
| Deletion Size | 21,531 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,009,036 | 78,009,054 | AACAGGGCAAGCACCACAG |
| TUP | 78,009,006 | 78,009,028 | AGTTGAGGGTGCACAGCCTCTTC |
| TUR | 78,008,963 | 78,008,981 | GATCCTCCCAAGCCCAGAC |
| TDF | 77,988,639 | 77,988,658 | CTGAGTGGAGAAGGGAGGAC |
| TDP | 77,988,614 | 77,988,636 | TGGGAGGCCCAGAACTTTCCAGG |
| TDR | 77,988,561 | 77,988,581 | CATTGAACGCATGGACAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TCAGTCAGGCTCAGGTTGTG |
| SD | TGCACCTGAGTCCTGACAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17449A-A10 | VGB6 | C57BL/6NTac | |
| 17449A-H1 | VGB6 | C57BL/6NTac | |
| 17449A-H10 | VGB6 | C57BL/6NTac | |
| 17449A-H11 | VGB6 | C57BL/6NTac | |
