| Gene | Pfkp |
| Chromosome | 13 |
| Deletion Start | 6,648,676 |
| Deletion End | 6,614,707 |
| Deletion Size | 33,970 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,647,842 | 6,647,861 | CGGGAAAGACCAGGAAGCAG |
| TUP | 6,647,816 | 6,647,836 | CCGGGACGTCATCAGCCCTCA |
| TUR | 6,647,797 | 6,647,815 | GAAGCCCGGAGCTGGTAAG |
| TDF | 6,615,112 | 6,615,133 | GCTCTAGCACAAAGGGAACAGG |
| TDP | 6,615,084 | 6,615,111 | CAACGTGGTTCTGTAGGCTAAACTGGGA |
| TDR | 6,615,053 | 6,615,072 | CCACAACAGCAAGCGAACAC |
PCR Primers
| Primer | Sequence |
| SU | TACCCGGCTATAATCCCTCC |
| SD | GAATGTGCTGGCTGCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17450A-A9 | VGB6 | C57BL/6NTac | |
| 17450A-B10 | VGB6 | C57BL/6NTac | |
