| Gene | Phospho2 |
| Chromosome | 2 |
| Deletion Start | 69,796,249 |
| Deletion End | 69,795,505 |
| Deletion Size | 745 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,795,717 | 69,795,735 | GCCCTTCACCTCAGGGATG |
| TUP | 69,795,744 | 69,795,772 | TCTCAGCTTCCTGAGGATGAATAAGGACA |
| TUR | 69,795,773 | 69,795,796 | CCGAAATGATGATGCAGTCAAATC |
| TDF | 69,795,932 | 69,795,952 | GCACTAGGTGCCCAAAGAATC |
| TDP | 69,795,955 | 69,795,979 | TGCAAAAATACAGTTTTGGGAGAAT |
| TDR | 69,796,034 | 69,796,057 | GGCAGACATCATTTCCACCATCAC |
PCR Primers
| Primer | Sequence |
| SU | GGTAAATTCTCATTGTATAGC |
| SD | TCCTATGGCCCTGATTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17451A-D1 | VGB6 | C57BL/6NTac | |
| 17451A-D11 | VGB6 | C57BL/6NTac | |
| 17451A-G6 | VGB6 | C57BL/6NTac | |
| 17451A-F7 | VGB6 | C57BL/6NTac | |
| 17451A-F1 | VGB6 | C57BL/6NTac | |
| 17451A-G7 | VGB6 | C57BL/6NTac | |
