| Gene | Plekha3 |
| Chromosome | 2 |
| Deletion Start | 76,695,821 |
| Deletion End | 76,675,598 |
| Deletion Size | 20,224 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,676,431 | 76,676,454 | CCAACCTCTGAATCCCATGATCTC |
| TUP | 76,676,457 | 76,676,480 | TGAACTAGAGGAAAACCGGCGGCT |
| TUR | 76,676,518 | 76,676,539 | GATGACAACTGCCCATCCTTTG |
| TDF | 76,694,905 | 76,694,924 | TGTGTTCCCTTGAGCATCAC |
| TDP | 76,694,925 | 76,694,948 | TGAAACCTGTGAGCCGTGTGGAAA |
| TDR | 76,694,988 | 76,695,008 | AGTAACAGCACAGAGCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GGAAGTGAAAGCGACGTC |
| SD | GCTCAATATGTCATGGTATTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17454A-B10 | VGB6 | C57BL/6NTac | |
| 17454A-D4 | VGB6 | C57BL/6NTac | |
| 17454A-D9 | VGB6 | C57BL/6NTac | |
| 17454A-H2 | VGB6 | C57BL/6NTac | |
| 17454A-H12 | VGB6 | C57BL/6NTac | |
