| Gene | Plp1 |
| Chromosome | X |
| Deletion Start | 136,836,143 |
| Deletion End | 136,822,917 |
| Deletion Size | 13,227 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,823,069 | 136,823,088 | TGCTGAGCATACTGCTTCAC |
| TUP | 136,823,089 | 136,823,114 | TGCCAACGAATCCTGGCAGTACATGT |
| TUR | 136,823,115 | 136,823,135 | TCCAAAGGTCTGGAAGACACC |
| TDF | 136,835,511 | 136,835,531 | GCTCCTTGTGCAGAGGTGAAG |
| TDP | 136,835,532 | 136,835,556 | CCTTAGGTGCCAGTGTGCAAGGTGA |
| TDR | 136,835,610 | 136,835,629 | TGCCGTACTGAGGAGGATTC |
PCR Primers
| Primer | Sequence |
| SU | GGAGCCCTTAAAGAAGGGAG |
| SD | CCAGGGACATTTCTGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17455A-A12 | VGB6 | C57BL/6NTac | |
| 17455A-B2 | VGB6 | C57BL/6NTac | |
| 17455A-B8 | VGB6 | C57BL/6NTac | |
| 17455A-C4 | VGB6 | C57BL/6NTac | |
| 17455A-D10 | VGB6 | C57BL/6NTac | |
