| Gene | Pms1 |
| Chromosome | 1 |
| Deletion Start | 53,282,069 |
| Deletion End | 53,256,632 |
| Deletion Size | 25,438 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,282,017 | 53,282,040 | CCTGTCCAGTTCTCAGACAATCAC |
| TUP | 53,281,991 | 53,282,015 | TCAGTGGTCAGCGTTGTGAAAGAGC |
| TUR | 53,281,929 | 53,281,952 | TGGGACACTCACCAGTTTAACTTC |
| TDF | 53,257,528 | 53,257,552 | GTGGGTAGATTGTAGGCTTAGATTG |
| TDP | 53,257,496 | 53,257,520 | CCTACAAGGTTTGGATCTAAAATAC |
| TDR | 53,257,458 | 53,257,478 | CTGCTGAGCTGTCCCTAGAAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGCTGTTGGGTCACAATG |
| SD | GTACAACTGTGGAATGTGGTAA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17456A-A5 | VGB6 | C57BL/6NTac | |
| 17456A-B11 | VGB6 | C57BL/6NTac | |
