| Gene | Polr3h |
| Chromosome | 15 |
| Deletion Start | 81,925,893 |
| Deletion End | 81,916,607 |
| Deletion Size | 9,287 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,925,132 | 81,925,151 | GGGCCTCGGTTTGTGTTATC |
| TUP | 81,925,106 | 81,925,131 | TGTGTTGCCCACAGTGTGAGAGTAGA |
| TUR | 81,925,083 | 81,925,102 | ACCCGCACGCTTATCCTCTC |
| TDF | 81,917,437 | 81,917,456 | CCCTCAGCCTCATGTACTCC |
| TDP | 81,917,412 | 81,917,434 | TGACACGATTGTGGCCTTGACGC |
| TDR | 81,917,382 | 81,917,402 | ACACTTGCTCCGCTTCATCAC |
PCR Primers
| Primer | Sequence |
| SU | GGGCAGATGCTTCTTATTGG |
| SD | AACCACCAAACAGTCCTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17457A-H12 | VGB6 | C57BL/6NTac | |
| 17457A-D8 | VGB6 | C57BL/6NTac | |
| 17457A-G5 | VGB6 | C57BL/6NTac | |
| 17457A-G7 | VGB6 | C57BL/6NTac | |
| 17457A-G10 | VGB6 | C57BL/6NTac | |
