| Gene | Pomt1 |
| Chromosome | 2 |
| Deletion Start | 32,254,496 |
| Deletion End | 32,236,709 |
| Deletion Size | 17,788 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,237,409 | 32,237,429 | GGCCTCTAGTGGTGACTGTTG |
| TUP | 32,237,434 | 32,237,462 | CAATTTGAACTTGGTAGCTCTGACTGGCC |
| TUR | 32,237,486 | 32,237,506 | AGCCCGAGGGTAGGAGAGTTG |
| TDF | 32,253,844 | 32,253,864 | ATCCTGCTGCTCCCGATTGTC |
| TDP | 32,253,865 | 32,253,885 | CTGCAGCACGCCAGCGACCAT |
| TDR | 32,253,904 | 32,253,926 | GGCTAAGCTCTTCCTGGTTTCAC |
PCR Primers
| Primer | Sequence |
| SU | CACCACTCACACAGCTCCAG |
| SD | TCAGGGTTTACAGAGGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17458A-H11 | VGB6 | C57BL/6NTac | |
| 17458A-D6 | VGB6 | C57BL/6NTac | |
| 17458A-D9 | VGB6 | C57BL/6NTac | |
| 17458A-E7 | VGB6 | C57BL/6NTac | |
| 17458A-G2 | VGB6 | C57BL/6NTac | |
| 17458A-E12 | VGB6 | C57BL/6NTac | |
