| Gene | Ppp1r16a |
| Chromosome | 15 |
| Deletion Start | 76,694,739 |
| Deletion End | 76,690,897 |
| Deletion Size | 3,843 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,691,136 | 76,691,157 | AAACGACTTGGAGGAAGGTGAG |
| TUP | 76,691,160 | 76,691,182 | TGCTTGAGTCCTAGGGCAGAGCA |
| TUR | 76,691,220 | 76,691,240 | CCTAGTTCCGAGCCAACACAG |
| TDF | 76,694,227 | 76,694,246 | GAAGCCCAAGGCTGGTGTAG |
| TDP | 76,694,248 | 76,694,271 | CGGAAACAAGCACCTCCTGATGGC |
| TDR | 76,694,290 | 76,694,308 | GGGCCATCGTCCTGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | CTGCCAGCTGAACACTTGAG |
| SD | AGGTGGAAGTTAAGGCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17461A-B3 | VGB6 | C57BL/6NTac | |
| 17461A-C7 | VGB6 | C57BL/6NTac | |
| 17461A-H9 | VGB6 | C57BL/6NTac | |
| 17461A-F10 | VGB6 | C57BL/6NTac | |
