| Gene | Ppp2r5b |
| Chromosome | 19 |
| Deletion Start | 6,234,828 |
| Deletion End | 6,228,370 |
| Deletion Size | 6,459 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,233,954 | 6,233,974 | GATGTGCCAGCCTCTGAGTTG |
| TUP | 6,233,927 | 6,233,952 | ATGAGTTGCTGAGCCGGAAACTGGCC |
| TUR | 6,233,888 | 6,233,908 | AGCCACGCAGTCCAAGAAGTC |
| TDF | 6,229,295 | 6,229,317 | TGAGTACATCCTGAGCCTCATTG |
| TDP | 6,229,271 | 6,229,294 | AGGACAACTGCCACACTGTGCTGC |
| TDR | 6,229,232 | 6,229,253 | GCTCCTTGGACACTTGGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTTTGGAAAGAACCGACCTG |
| SD | CCTGGACAAGGTCTTGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17462A-B1 | VGB6 | C57BL/6NTac | |
| 17462A-B4 | VGB6 | C57BL/6NTac | |
| 17462A-B11 | VGB6 | C57BL/6NTac | |
| 17462A-D12 | VGB6 | C57BL/6NTac | |
| 17462A-F6 | VGB6 | C57BL/6NTac | |
| 17462A-B2 | VGB6 | C57BL/6NTac | |
