| Gene | Pppde2 |
| Chromosome | 15 |
| Deletion Start | 82,015,962 |
| Deletion End | 81,994,665 |
| Deletion Size | 21,298 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,015,636 | 82,015,657 | GCCGACTGATCTTGGTGGATTC |
| TUP | 82,015,613 | 82,015,634 | CCAAGAAGCCAGGGAACCCTGC |
| TUR | 82,015,565 | 82,015,584 | GGACACGAGCTGGAGGTATC |
| TDF | 81,995,463 | 81,995,487 | GCAGAAGACGGGATAGAAAGAGTTG |
| TDP | 81,995,437 | 81,995,462 | AAGAGCCAGAGGCAGTGGACAGCTTA |
| TDR | 81,995,385 | 81,995,404 | TGCGTGCCTATGCTCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGTTCCCTAAACTGGAACGC |
| SD | CTTGAGTGAGGAGCCTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17463A-A3 | VGB6 | C57BL/6NTac | |
| 17463A-B2 | VGB6 | C57BL/6NTac | |
| 17463A-B5 | VGB6 | C57BL/6NTac | |
| 17463A-E10 | VGB6 | C57BL/6NTac | |
| 17463A-H11 | VGB6 | C57BL/6NTac | |
