| Gene | Prdm4 |
| Chromosome | 10 |
| Deletion Start | 85,912,967 |
| Deletion End | 85,893,071 |
| Deletion Size | 19,897 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,912,803 | 85,912,821 | CCGCTGCCCTTGAACATTG |
| TUP | 85,912,771 | 85,912,799 | TCAGTTCTGAGTGTGCTAAGGGTAGTACA |
| TUR | 85,912,753 | 85,912,770 | GGCACTGGCTCGTTTCTC |
| TDF | 85,893,286 | 85,893,308 | AGTACCATCTCACTCGCCACCTG |
| TDP | 85,893,261 | 85,893,284 | AGACCTGCAAAGAGCCCAGCTCCA |
| TDR | 85,893,221 | 85,893,243 | TCCTCCGACTCATCATCCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | TGTGTTGATAAGCCAAATGGAG |
| SD | AGTGGGATGGCTCAAGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17464A-B1 | VGB6 | C57BL/6NTac | |
| 17464A-D5 | VGB6 | C57BL/6NTac | |
| 17464A-H3 | VGB6 | C57BL/6NTac | |
