| Gene | Prorsd1 |
| Chromosome | 11 |
| Deletion Start | 29,514,997 |
| Deletion End | 29,512,925 |
| Deletion Size | 2,073 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,514,817 | 29,514,835 | AAACTGCGGCAACCGTGAG |
| TUP | 29,514,788 | 29,514,815 | AAACTTGTCCTGGGATGACTGACGTGAC |
| TUR | 29,514,729 | 29,514,753 | TGTAGTGCCTTGTTCCTTAGTTATC |
| TDF | 29,513,361 | 29,513,383 | TCTGGAAGGTGGACATGAGAAAG |
| TDP | 29,513,332 | 29,513,360 | TGTACTTTCACCCAATGACTAATGCTGCA |
| TDR | 29,513,280 | 29,513,304 | GTCCTGTAGCCTTCACAAATATGAG |
PCR Primers
| Primer | Sequence |
| SU | GAACGGAAAGACGGAAGTC |
| SD | CATCTGAACCAGTTCTGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17466A-A5 | VGB6 | C57BL/6NTac | |
| 17466A-A9 | VGB6 | C57BL/6NTac | |
| 17466A-B1 | VGB6 | C57BL/6NTac | |
| 17466A-C3 | VGB6 | C57BL/6NTac | |
| 17466A-G3 | VGB6 | C57BL/6NTac | |
| 17466A-A4 | VGB6 | C57BL/6NTac | |
