| Gene | Prickle3 |
| Chromosome | X |
| Deletion Start | 7,667,808 |
| Deletion End | 7,659,615 |
| Deletion Size | 8,194 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,660,196 | 7,660,215 | TGAGCATTCGCCACTTGTTG |
| TUP | 7,660,217 | 7,660,242 | ACACGTCTAGGCACTGAGGACAGACA |
| TUR | 7,660,260 | 7,660,277 | CCATGCCCTCAGCCTTTG |
| TDF | 7,667,472 | 7,667,491 | GCCACCATCGCTGTGACTTG |
| TDP | 7,667,496 | 7,667,525 | CAGGTTCAGATTCAGGATCTTGCTCCAGCT |
| TDR | 7,667,548 | 7,667,571 | AAGAAGCCATCATCCTCAGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGGTCACAAGGCAAAGTCAG |
| SD | CTGCCAATCAACCCATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17467A-B5 | VGB6 | C57BL/6NTac | |
| 17467A-F8 | VGB6 | C57BL/6NTac | |
