| Gene | Prkcsh |
| Chromosome | 9 |
| Deletion Start | 22,013,158 |
| Deletion End | 22,003,462 |
| Deletion Size | 9,697 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,003,798 | 22,003,816 | GCGTTTGCATTTGCCCATC |
| TUP | 22,003,818 | 22,003,843 | CCCATTAAATGAGCCTGCTAAGCGCA |
| TUR | 22,003,871 | 22,003,892 | ACTGAGCAGGAAGCAACAGGAG |
| TDF | 22,013,065 | 22,013,084 | CCAGTCGCTGTGAGTACCTC |
| TDP | 22,013,086 | 22,013,110 | TGGAGCTGATGACACCAGCAGCCTG |
| TDR | 22,013,125 | 22,013,144 | CCCATCACTGGGTGCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTTTACCGAGAGACCTATGG |
| SD | AATAAAGAAGCCGCAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17468A-A11 | VGB6 | C57BL/6NTac | |
| 17468A-C3 | VGB6 | C57BL/6NTac | |
| 17468A-D3 | VGB6 | C57BL/6NTac | |
| 17468A-F6 | VGB6 | C57BL/6NTac | |
| 17468A-G3 | VGB6 | C57BL/6NTac | |
| 17468A-H5 | VGB6 | C57BL/6NTac | |
