| Gene | Prmt7 |
| Chromosome | 8 |
| Deletion Start | 106,251,681 |
| Deletion End | 106,212,480 |
| Deletion Size | 39,202 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,212,794 | 106,212,813 | CTTGGAGGAGCAGGAAGGAG |
| TUP | 106,212,815 | 106,212,840 | TTGGTAACCCACTGAATGCAGCAAGA |
| TUR | 106,212,861 | 106,212,879 | CTCGCCGGGAGGAATCTTG |
| TDF | 106,251,165 | 106,251,183 | TGCTGACTGCTTGGCTCTG |
| TDP | 106,251,187 | 106,251,208 | TCCCTCCCAGACCCGGGAAGAG |
| TDR | 106,251,246 | 106,251,264 | TGCTGATCGTGCTGTCTGG |
PCR Primers
| Primer | Sequence |
| SU | GTCAGAAATATCAAAATACTGG |
| SD | TGCACTGAGGACACAAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17469A-B11 | VGB6 | C57BL/6NTac | |
| 17469A-D6 | VGB6 | C57BL/6NTac | |
| 17469A-E11 | VGB6 | C57BL/6NTac | |
| 17469A-F3 | VGB6 | C57BL/6NTac | |
