| Gene | Pskh1 |
| Chromosome | 8 |
| Deletion Start | 105,930,021 |
| Deletion End | 105,912,693 |
| Deletion Size | 17,329 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,913,017 | 105,913,034 | AGCCGAGTGGTACGTGTG |
| TUP | 105,913,036 | 105,913,054 | AGCACCGGGCAACCCGACA |
| TUR | 105,913,067 | 105,913,091 | TCGGTACTTGGTCTCTATCATCTTG |
| TDF | 105,929,370 | 105,929,388 | AGCCAGCCTTTGGGTGTAG |
| TDP | 105,929,394 | 105,929,421 | TTAAGTTCCCTGCCTAGGGTAGATGACA |
| TDR | 105,929,440 | 105,929,457 | TTGATGCCTGGTGGAGTC |
PCR Primers
| Primer | Sequence |
| SU | CATCCTGAGAGGTGAAAGGC |
| SD | GCCATAGGACCCTTCTACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17470A-B8 | VGB6 | C57BL/6NTac | |
| 17470A-C12 | VGB6 | C57BL/6NTac | |
| 17470A-F8 | VGB6 | C57BL/6NTac | |
| 17470A-H8 | VGB6 | C57BL/6NTac | |
| 17470A-D1 | VGB6 | C57BL/6NTac | |
