| Gene | Psmc3 |
| Chromosome | 2 |
| Deletion Start | 91,059,464 |
| Deletion End | 91,054,251 |
| Deletion Size | 5,214 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,054,627 | 91,054,646 | AGCGCACACGGCTGTTAGAC |
| TUP | 91,054,651 | 91,054,674 | AGATCAAGGTGGGCTCCTGGCTCC |
| TUR | 91,054,710 | 91,054,729 | AGCCTCGGGTTCCTCATCTG |
| TDF | 91,058,986 | 91,059,007 | CTTCCCTTTGCTGTTCCCTCTC |
| TDP | 91,059,008 | 91,059,037 | CGTGACTAATCAAGCTCTGTGGTGTCTTGT |
| TDR | 91,059,058 | 91,059,078 | CACCGAGCCAGCTCTTCATAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCATTTCCCGAAAGACTC |
| SD | GGATCTTGCTAAAGGTTAGTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17471A-A6 | VGB6 | C57BL/6NTac | |
| 17471A-A7 | VGB6 | C57BL/6NTac | |
| 17471A-E1 | VGB6 | C57BL/6NTac | |
