| Gene | Ptgds |
| Chromosome | 2 |
| Deletion Start | 25,469,670 |
| Deletion End | 25,467,302 |
| Deletion Size | 2,369 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,469,522 | 25,469,542 | CCCACACCCAAAGAAGCAGAG |
| TUP | 25,469,499 | 25,469,521 | CCCTCAGGCCAGAGCCAGGCTTT |
| TUR | 25,469,463 | 25,469,481 | GGCTGACAGTGCCCAGAAC |
| TDF | 25,468,142 | 25,468,161 | TGGTGGAGGCCAACTATGAC |
| TDP | 25,468,107 | 25,468,131 | TGCTATTCAGCAGAGGCACCAAGGG |
| TDR | 25,468,054 | 25,468,073 | GTGGGCTGGGATACCTACTG |
PCR Primers
| Primer | Sequence |
| SU | GCAGGCAGAGGTTTCTTC |
| SD | AATGGCACTAGTGTGGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17473A-A9 | VGB6 | C57BL/6NTac | |
| 17473A-A10 | VGB6 | C57BL/6NTac | |
| 17473A-B12 | VGB6 | C57BL/6NTac | |
