| Gene | Ptges2 |
| Chromosome | 2 |
| Deletion Start | 32,402,389 |
| Deletion End | 32,396,278 |
| Deletion Size | 6,112 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,396,788 | 32,396,805 | GGAACGAGTGACCCTGAC |
| TUP | 32,396,807 | 32,396,831 | TCAGACTCAGAACTGGAGGCGTGCT |
| TUR | 32,396,879 | 32,396,897 | GCGGAGAAGGGCTAAAGAC |
| TDF | 32,402,306 | 32,402,325 | TCAACCCTAGCTGCAAAGAC |
| TDP | 32,402,330 | 32,402,355 | TGCTAGGGACACAAGACCTGGACCAG |
| TDR | 32,402,365 | 32,402,384 | ACTCTGCCCTCTGTGAGCAC |
PCR Primers
| Primer | Sequence |
| SU | ATCGTAGATGCACGATGTGG |
| SD | ACCATTGTCAGACCAGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17474A-B3 | VGB6 | C57BL/6NTac | |
| 17474A-C10 | VGB6 | C57BL/6NTac | |
| 17474A-E4 | VGB6 | C57BL/6NTac | |
| 17474A-E1 | VGB6 | C57BL/6NTac | |
| 17474A-D6 | VGB6 | C57BL/6NTac | |
