| Gene | Pttg1ip |
| Chromosome | 10 |
| Deletion Start | 77,597,100 |
| Deletion End | 77,581,825 |
| Deletion Size | 15,276 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,582,430 | 77,582,450 | CACTCCTGCTACCTTGGTGAG |
| TUP | 77,582,453 | 77,582,476 | TGACTAAAGGAGCTGACCCTGGCC |
| TUR | 77,582,507 | 77,582,527 | TGCCTGGCATAAAGTCCTTAC |
| TDF | 77,596,182 | 77,596,204 | GGGAGATGCGAATAAGTGGGAAG |
| TDP | 77,596,211 | 77,596,235 | AAGAGAAAAGTGGGTGGGTGCCCCA |
| TDR | 77,596,282 | 77,596,304 | GCACATGCCATCAAGTTGAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CTCCATTCCAGAGCAAGTGG |
| SD | AGGGTGGTGGTCAGTTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17475A-A2 | VGB6 | C57BL/6NTac | |
| 17475A-A3 | VGB6 | C57BL/6NTac | |
| 17475A-A5 | VGB6 | C57BL/6NTac | |
| 17475A-C11 | VGB6 | C57BL/6NTac | |
| 17475A-D7 | VGB6 | C57BL/6NTac | |
| 17475A-D4 | VGB6 | C57BL/6NTac | |
