| Gene | Pwp1 |
| Chromosome | 10 |
| Deletion Start | 85,888,228 |
| Deletion End | 85,871,946 |
| Deletion Size | 16,283 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,872,544 | 85,872,566 | GGGTTGCTATGGGTGTAATTGTC |
| TUP | 85,872,574 | 85,872,602 | TGACAACTCGATATGTGATAGACGTGGGA |
| TUR | 85,872,611 | 85,872,634 | CCTCACCGCATGTAAATTCATGTC |
| TDF | 85,887,357 | 85,887,381 | TGAAAGCATAAGGACTAAGAACAGG |
| TDP | 85,887,383 | 85,887,410 | CTGGGATGTTACGTACATGGCAAAGGCA |
| TDR | 85,887,434 | 85,887,455 | GCCCACCGCATTTCTAAGTAAG |
PCR Primers
| Primer | Sequence |
| SU | GGAAGCAGTCGTAGATTTGC |
| SD | GCTAACACAGCAAGACCCGT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17476A-B2 | VGB6 | C57BL/6NTac | |
| 17476A-C4 | VGB6 | C57BL/6NTac | |
| 17476A-C7 | VGB6 | C57BL/6NTac | |
| 17476A-G10 | VGB6 | C57BL/6NTac | |
| 17476A-G9 | VGB6 | C57BL/6NTac | |
| 17476A-E6 | VGB6 | C57BL/6NTac | |
