| Gene | Rabl3 |
| Chromosome | 16 |
| Deletion Start | 37,571,108 |
| Deletion End | 37,539,918 |
| Deletion Size | 31,191 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,539,930 | 37,539,950 | CGGGTGAAGGTTCTGGTGTTG |
| TUP | 37,539,953 | 37,539,977 | AGACTCAGGTGAGCCGCTTAACCCA |
| TUR | 37,539,985 | 37,540,002 | TCGGGCGTGTTGGTAGTC |
| TDF | 37,570,985 | 37,571,008 | TGACTCTTTCTGCTACGGCATAAC |
| TDP | 37,571,014 | 37,571,039 | TCTTTCTTGCCTACAGATTCCGGGCT |
| TDR | 37,571,072 | 37,571,095 | TCAGTCACAGTGGAAGTTCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | GAGGACCAAAGCAAGAGGTG |
| SD | CCGCTGGTTCCAAGTAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17479A-D4 | VGB6 | C57BL/6NTac | |
| 17479A-D5 | VGB6 | C57BL/6NTac | |
| 17479A-E11 | VGB6 | C57BL/6NTac | |
