| Gene | Rhod |
| Chromosome | 19 |
| Deletion Start | 4,439,348 |
| Deletion End | 4,426,050 |
| Deletion Size | 13,299 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,438,944 | 4,438,962 | TTGGCGACGAGAGTGTGAG |
| TUP | 4,438,920 | 4,438,942 | ACTTTGGAGCTCGACTGGCAGCA |
| TUR | 4,438,892 | 4,438,912 | GCTGTAAGAGGCAAAGGCTTG |
| TDF | 4,426,207 | 4,426,228 | CGGTGGCCTATCTTGAGTGTTC |
| TDP | 4,426,183 | 4,426,206 | AGCTCGGCTCCATGACAACGTGGA |
| TDR | 4,426,131 | 4,426,151 | GTTATGTCTGCGACTGCTGAG |
PCR Primers
| Primer | Sequence |
| SU | TGTCTACTCTCCCACGAGCC |
| SD | AATAGGATGATCACGGACGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17486A-H12 | VGB6 | C57BL/6NTac | |
| 17486A-E6 | VGB6 | C57BL/6NTac | |
| 17486A-E9 | VGB6 | C57BL/6NTac | |
| 17486A-A11 | VGB6 | C57BL/6NTac | |
