| Gene | Rnf130 |
| Chromosome | 11 |
| Deletion Start | 50,052,965 |
| Deletion End | 50,025,404 |
| Deletion Size | 27,562 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,025,877 | 50,025,898 | TTTACTGGAGGGCACTGGAATG |
| TUP | 50,025,904 | 50,025,931 | TTTCAGGGACAGTTGAGACAAAGGAGGT |
| TUR | 50,025,952 | 50,025,971 | GCATCTCGTCCACCATGAAC |
| TDF | 50,052,635 | 50,052,655 | TGCATTGTGTTGCCCTTTGTG |
| TDP | 50,052,657 | 50,052,682 | AAATCCATACAGCCCTGCTGCAGTGA |
| TDR | 50,052,699 | 50,052,721 | GTGTCCTTCTGTTCCCTGAATAC |
PCR Primers
| Primer | Sequence |
| SU | ACCACTCCGAGTCCTCTCA |
| SD | AACTACTGTGAGCTCCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17489A-D5 | VGB6 | C57BL/6NTac | |
| 17489A-C12 | VGB6 | C57BL/6NTac | |
