| Gene | S100b |
| Chromosome | 10 |
| Deletion Start | 76,260,110 |
| Deletion End | 76,257,038 |
| Deletion Size | 3,073 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,257,386 | 76,257,405 | ACGTCAGCACGCATCTGTAG |
| TUP | 76,257,406 | 76,257,433 | TCCTTGTACTTCAAAGGTGGAAGCAGGA |
| TUR | 76,257,461 | 76,257,479 | GTTCTCACCGGCCTTGTTC |
| TDF | 76,259,810 | 76,259,830 | TTCCTGTATTGGGACTGCTTC |
| TDP | 76,259,831 | 76,259,854 | TGACGCAAATCGATCGGCCTGGTA |
| TDR | 76,259,867 | 76,259,884 | AAGACGGAGCCGTCAGAG |
PCR Primers
| Primer | Sequence |
| SU | AATAGCCACCAGGAAGACCC |
| SD | AAGGATGAGCAACCTCTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17492A-A9 | VGB6 | C57BL/6NTac | |
| 17492A-B1 | VGB6 | C57BL/6NTac | |
| 17492A-D1 | VGB6 | C57BL/6NTac | |
| 17492A-F8 | VGB6 | C57BL/6NTac | |
| 17492A-E8 | VGB6 | C57BL/6NTac | |
